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mAbs Against HUMAN Protein

CSF1R

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:FER216
PROTEIN:Macrophage colony-stimulating factor 1 receptor precursor
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/NP_005202.2
ANTIGEN USED:ecCSF1R-Fc-6His recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. [provided by RefSeq, Dec 2013].

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Martín-Moreno AM, Roncador G, Maestre L, Mata E, Jiménez S, Martínez-Torrecuadrada JL, Reyes-García AI, Rubio C, Tomás JF, Estévez M, Pulford K, Piris MA, García JF. CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma. PLoS One 2015 Jun 12;10(6).

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
FER216 Neat supernatant Tonsil /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
FER216 1:10 supernatant Tonsil / Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
FER216 supernatant Tonsil / Available
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FER216 mAb in transfected cells
Validation of FER216 monoclonal antibody in HEK293T/CSF1R transfected cells. HEK293T/FGFR1 transfected cells were used as negative control. anti-CSF1R antibody (clone 61710) confirms transfection efficiency.
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FER216 mAb in human frozen tonsil section
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FER216 staining in human paraffin embeded tissues
The range of expression of CSF1R in tisues varied considerably between the different functional tissue compartments. The higher frequency of CSF1R+ macrophages was detected in the paracortex of tonsil.
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Immunofluorescence of FER216 mAb in human paraffin embeded tonsil.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
FER216 1:2 supernatant Tonsil / L540 cell line Available
Immunoprecipitation
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FER216 mAb in western blotting
Lane1 HEK-CSF1R-Fc transfected cells (20ug) (+)
Lane 2 HEK-FGFR1 transfected cells (20ug) (-)
Lane 3 Human tonsil (150ug) (+)
Lane 4 Human testicle (150ug) (-)
Lane 5 Hodgking lymphoma case (150ug) (+)
Lane 6 Hodgking lymphoma case (150ug) (+)
Lane 7 Hodgking lymphoma case (150ug) (+)
Lane 8 L428 cell line (150ug)(-)
Lane 9 L1236 cell line (150ug) (-)
Lane 10 KM-H2 cell line (150ug) (-)
Lane 11 HDLM2 cell line (150ug) (-)
Lane 12 L540 cell line (150ug) (-)
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Immunoprecipitation with FER216
Immunoprecipitation of protein extracts from human tonsil, one HD case, KMH2, and L540 with FER216 mAb (30ul/IP) followed by western blotting with FER216 (1:5 dilution).
FLOW CYTOMETRY
FLOW CYTOMETRY Clone Dilution +/- control Type of fluorocrom Protocol
Recommended Result obtained is satisfactory. The reagent can be use in this application
FER216 / PE
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Expression of CSF1R (FER216) on Karpas 299 human cell line.