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Antibody valitation: Practical guide to finding and validating suitable antibodies for research

3.C Decide whether further validation is necessary

No: Sufficient existing validation data is available that shows the antibody is suitable for my experimental conditions. Only optimisation of the antibody is required to obtain the best performance in my work.

Yes: Insufficient data is present to determine that the antibody has been appropriately validated. Decide and perform the additional validation listed above required using the relevant positive and negative controls and techniques.