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Friedrich Koch-Nolte

Name of the laboratory

Laboratory of Molecular Immunology
Institute of Immunology
University Medical Center Hamburg-Eppendorf
Martinistr. 42
20246 Hamburg, Germany

Members of the laboratory

Members: Laboratory of Molecular Immunology
Unit Leader
Friedrich Koch-Nolte
Research Fellows
Stephan Menzel
Nicole Schwarz
Thomas Eden
Anna Eichhoff
Mirjam Loew
Fabienne Seyfried
Gudrun Dubberke
Birte Albrecht
Ph.D. Students
Carolina Pinto
Thomas Eden
Anna Eichhoff
Intissar Chachbani
Tobias Schaefer
Julia Hambach

Research activities

The focus of our laboratory is on the molecular characterization of lymphocyte membrane proteins, in particular receptors and enzymes involved in signaling by extracellular nucleotides. We generate monoclonal antibodies and nanobodies - single domain antibodies derived from camelid heavy chain antibodies - as new research and therapeutic tools. We are interested in ADP-ribosylation as a pathogenic mechanism of bacterial toxins and as a reversible posttranslational modification regulating protein functions. Using genetic immunization and antibody engineering, we strive to develop new tools for combating infections and for treating diseases of the immune system. Many of our monoclonal and recombinant antibodies are commercialized via MediGate GmbH, a 100% subsidy of the University Medical Center Hamburg-Eppendorf.

Techniques available

  • Monoclonal antibody production in rats and mice
  • Polyclonal antibody production
  • Genetic immunization
  • Nanobody production from llamas and dromedaries
  • Recombinant antibody production in HEK cells
  • Cloning techniques
  • Antibody characterization and conjugation
  • Mycoplasma testing
  • Flow cytometry, ELISA, immunofluorescence microscopy, etc.

Publications (2018-present)

  • The ABCD database: a repository for chemically defined antibodies. Lima WC, Gasteiger E, Marcatili P, Duek P, Bairoch A, Cosson P. Nucleic Acids Res. 2019 Aug 14. pii: gkz714. doi: 10.1093/nar/gkz714. [Epub ahead of print] PMID:31410491
  • Eden T, Menzel S, Wesolowski J, Bergmann P, Nissen M, Dubberke G, Seyfried F, Albrecht B, Haag F, Koch-Nolte F. (2018) A cDNA Immunization Strategy to Generate Nanobodies against Membrane Proteins in Native Conformation. Front Immunol. 8:1989.
  • Exploiting Nanobodies' Singular Traits. Ingram JR, Schmidt FI, Ploegh HL. Annu Rev Immunol. 2018 Apr 26;36:695-715. doi: 10.1146/annurev-immunol-042617-053327. PMID: 29490163


  • Stortelers C, Pinto-Espinoza C, Van Hoorick D, Koch-Nolte F. (2018) Modulating ion channel function with antibodies and nanobodies. Curr Opin Immunol. 52:18-26.
  • Bannas P, Hambach J, Koch-Nolte F. (2017) Nanobodies and nanobody-based human heavy chain antibodies as antitumor therapeutics. Front Immunol. 8:1603.
  • Rissiek B, Koch-Nolte F, Magnus T. (2014) Nanobodies as modulators of inflammation: potential applications for acute brain injury. Front Cell Neurosci. 8:344.
  • Menzel S, Rissiek B, Haag F, Goldbaum F, Koch-Nolte F. (2013) The art of blocking ARTs: Nanobodies as experimental and therapeutic tools to block mammalian and toxin ADP-ribosyltransferases. FEBS J. 280:3543-50.
  • Wesolowski J, Alzogaray V, Reyelt J, Unger M, Juarez K, Urrutia M, Cauerhff A, Danquah W, Rissiek B, Scheuplein F, Schwarz N, Adriouch S, Boyer O, Seman M, Licea A, Serreze DV, Goldbaum FA, Haag F, Koch-Nolte F (2009) Single domain antibodies: promising experimental and therapeutic tools in infection and immunity. (review) Med Microbiol Immunol 198:157-74.
  • Koch-Nolte F, Haag F, Guse AH, Lund F, Ziegler M. (2009) Emerging roles of NAD+ and its metabolites in cell signaling. Sci Signal. 10:mr1.
  • Koch-Nolte F, Kernstock S, Mueller-Dieckmann C, Weiss MS, Haag F. (2008) Mammalian ADP-ribosyltransferases and ADP-ribosylhydrolases.  Front Biosci. 2008 May 1;13:6716-29.