Name of the laboratory
- Department of Immunology and Biotechnology
- Clinical Center, University of Pecs
- H-7634 Pecs, Szigeti ut 12
Members of the laboratory
- Group leader
Dr. Peter Balogh, MD, PhD email@example.com
- Postdoctoral scientist/resident
Dr. Zoltan Kellermayer, MD firstname.lastname@example.org
- PhD student
Dora Vojkovics Medical Biotechnology MSc email@example.com
- Research associate
Diana Heidt firstname.lastname@example.org
- Research technicians
Anett Gyöngyösi email@example.com
Janos Appl firstname.lastname@example.org
The Department of Immunology and Biotechnology (formerly "Immunological and Biotechnological Laboratory") was established in 1991 by Dr. Peter Nemeth as an independent teaching, research and diagnostic unit of the University Medical School of Pécs. This unit was dedicated to pursue its own research in basic and applied immunology, particularly to produce monoclonal antibodies for research and diagnostic purposes, now headed by Dr. Timea Berki.
The research interest of Dr Peter Balogh’s group focuses on the structure, organization and developmental features of the stromal constituents of peripheral lymphoid tissues and their role in lymphocyte homeostasis, particularly the role of Nkx2-3 transcription factor in the spleen and Peyer’s patches of mice. In this work several rat monoclonal antibodies have been generated against both hematopoietic and stromal cells (fibroblastic reticular cells, marginal zone macrophages, various endothelial subsets). Current investigations are conducted to study the impact of Nkx2-3 on the onset of inflammatory bowel diseases in various murine models and the mechanism of lymphoid tissue-specific tropism in B-cell lymphoma progression.
Analysis of stromal constituents in either mouse of human lymphoid tissues significantly lags behind the extensive knowledge concerning the diverse spectrum of hematopoietic elements, despite recent advances using lineage tracing and fate mapping experiments. Furthermore, it remains questionable to what extent the developmental and functional characteristics in mice can be relevant to humans. Recently Nkx2-3 has emerged as an important element in organizing the stromal scaffolding in mouse spleen and Peyer’s patches, and it may also influence perturbed gut immunity in humans leading to various forms of inflammatory bowel diseases. To study the role of Nkx2-3 transcription factor, Nkx2-3-deficient mice (developed by Oliver Pabst at the Technical University of Braunschweig) have been used in various compound mutations (Ltbr-/-, Rag2-/- and now in variants allowing lymphocyte distribution analysis through Luc and photoconvertible KikGR detection) are investigated. We found that the absence of this factor essentially reprograms the spleen and Peyer’s patches vasculature towards lymph node-like pattern, including the formation of ectopic HEVs expressing PNAd and abortive LYVE-1-positive lymphatic cysts. In this work we use multiparametric flow cytometry, adoptive cell transfer and bone marrow chimeric animals as well as four-color immunofluorescent analysis of the mucosal lymphoid compartments.
- Rat and mouse monoclonal antibody production, antibody purification and labeling (FITC, Cy dyes, biotinylation, HRPO), immunoprecipitation, Western blot, ELISA;
- Multicolor (up to 4 colors) immunofluorescence, immunohistochemistry (PO, AP and dual labeling), lectin-immunohistochemistry;
- Flow cytometry and sorting (FACSCalibur, FACSCanto, FACSAria);
- Lymphocyte adoptive transfer and tracing, bone marrow radiation chimeras, interspecies (rat x mouse) chimeras, Luc detection and access to bioluminescent imager, and KikGR photoconversion.
- Kellermayer Z, Mihalj M, Lábadi Á, Czömpöly T, Lee M, O'Hara E, Butcher EC, Berta G, Balogh A, Arnold HH, Balogh P. Absence of Nkx2-3 homeodomain transcription factor reprograms the endothelial addressin preference for lymphocyte homing in Peyer's patches. J Immunol. 2014 193: 5284-93.
- Kellermayer Z, Fisi V, Mihalj M, Berta G, Kóbor J, Balogh P. Marginal Zone Macrophage Receptor MARCO Is Trapped in Conduits Formed by Follicular Dendritic Cells in the Spleen. J Histochem Cytochem. 2014 62:436-449.
- Mihalj M, Kellermayer Z, Balogh P. Follicles in gut-associated lymphoid tissues create preferential survival niches for follicular Th cells escaping Thy-1-specific depletion in mice. Int Immunol. 2013 25:423-35.
- Shah AA, Mihalj M, Ratkay I, Lubka-Pathak M, Balogh P, Klingel K, Bohn E, Blin N, Baus-Loncar M. Increased susceptibility to Yersinia enterocolitica Infection of Tff2 deficient mice. Cell Physiol Biochem. 2012 30:853-62.
- Szarka E, Neer Z, Balogh P, Adori M, Angyal A, Prechl J, Kiss E, Kövesdi D, Sármay G. Exacerbation of collagen induced arthritis by Fcγ receptor targeted collagen peptide due to enhanced inflammatory chemokine and cytokine production. Biologics. 2012 6:101-15.
- CroatiaStipan JonjicUniversity of Rijeka
- FranceAndré PèlegrinInstitut de Recherche en Cancérologie de Montpellier, IRCM
- FranceArmand BensussanHôpital Saint Louis
- FrancePierre LafayeInstitut Pasteur
- GermanyFriedrich Koch-NolteUniversity Medical Center Hamburg-Eppendorf
- HungaryJohanna SymmonsEnglish & Scientific Consulting Kft. (SCICONS)
- HungaryPeter BaloghClinical Center, University of Pecs
- ItalyFabio MalavasiUniversity of Torino Medical School
- RussiaAlexander FilatovInstitute of Immunology
- RussiaAlexander TaraninInstitute of Molecular and Cellular Biology
- SloveniaVladka Čurin ŠerbecBlood Trasfusion Centre of Slovenia
- SpainGiovanna RoncadorCentro Nacional de Investigaciones Oncológicas
- SpainJosé I. Rodríguez BarbosaEdificio Nuevos Institutos de Investigación
- SpainLeonor KremerCentro Nacional de Biotecnología/CSIC
- SpainNúria PascualNb4D
- SpainPablo EngelMedical School, University of Barcelona
- United KingdomUniversity of OxfordLRF Haemato-oncology Group
- United KingdomVertebrate Antibodies Limited (VAL)
- United KingdomUniversity of OxfordOxFabs
- United KingdomKing's College LondonSt. John's Institute of Dermatology