Name of the laboratory
- Antibody engineering Platform
- Centre d’Innovation et de Technologie-CITECH
- Institut Pasteur
- 25-28, rue du Dr Roux
- 75015 Paris, France
- 33 1 40613920
Members of the platform
- Unit Leader
Pierre Lafaye email@example.com
- Research Engineer
François Traincard firstname.lastname@example.org
Sylvie Dartevelle email@example.com
Françoise Marchand firstname.lastname@example.org
Matthias Vandesquille email@example.com
Muriel Esnard firstname.lastname@example.org
The Platform is mostly devoted to the obtaining of antibodies useful for the pasteurian community. Our activities are focused on murine monoclonal antibodies (mAb) and camel homodimeric antibodies (VHH or NanobodiesTM).
There is a still a large demand for mAbs but we focus the activity on antigens such as polysaccharides, membrane proteins, unstable proteins, on which we gained experience, or on projects requiring close collaboration between teams to select the « right » antibodies by using specific screening which can only be performed by the demanding laboratory.
In addition, the Platform has acquired international reputation for the development of dipsticks for the diagnosis of cholera, plague, meningitis and bloody diarrheas. The technology is indeed well adapted for field diagnosis of tropical diseases, owing to its low cost, simplicity and insensitivity to rugged conditions. The principle of such dipsticks is shown in Fig. 2. As the dipstick is dipped in the biological fluid to be tested, antigen-antibody complexes are formed with the first antibody, which is conjugated to colloidal gold particles. The complexes then migrate by capillarity until they reach the line of immobilized capture antibody, which becomes materialized as a red line. A second line of immobilized rabbit anti-mouse Ig catches gold-conjugated antibodies that did not bind to the first line, and serves as a control for dipstick functioning.
VHHs are complimentary of mAbs in the sense that we demonstrated they are able to recognize non-conventional epitopes, to cross the BBB (Blood-Brain Barrier) and to bind an intracellular antigen. VHHs are useful either for brain imaging, viral neutralization and/or for cristallization studies.
The use of antibodies to detect intracerebral targets in vivo is limited by their reduced ability to cross the blood brain barrier (BBB). VHH, thanks to their small size, their stability and their basic isoelectric point, have the potential to transmigrate across the BBB and could be used as biomarkers after appropriate labeling.
As a proof of concept we have generated VHH against human glial fibrillary acidic protein (GFAP), and we have shown that this VHH E9 was able to cross the BBB following intra-arterial infusion, to diffuse in the brain, to penetrate into astrocytes and to label specifically GFAP (Li et al, Faseb J, 2012).
Then, we have engineered VHHs with an extra cysteine introduced in their C-terminal part to obtained site-specific conjugates. This strategy is particularly appealing for nanoprobes targeting intracerebral antigen as the conjugation should not affect the efficiency of the conjugates to cross the BBB and to reach their intracerebral targets. VHHs specific of the two main features of Alzheimer’s Disease (Aβ for extracellular amyloid plaques and p-Tau for intracellular neurobrillary tangles) have been labeled with fluorescent probes (i.e. AlexaFluor 488) and tested on mouse models of Alzheimer's Disease. The fluorescent VHHs were injected intravenously in the transgenic mice (PS2APP model of amyloidosis or rTg4510 model of tauopathy) and followed by in vivo two-photon microscopy. Very prominent staining of amyloid plaques or NFTs was observed suggesting both anti-Aβ VHH R3VQ and anti p-Tau VHH A2 were capable of passing through the BBB and then reach their specific targets in the brain parenchyma. These outcomes were further confirmed by immunohistochemistry.
Our results demonstrate that three VHHs directed against three different intracerebral targets were able to cross the BBB in vivo after intravenous injection. Their brain uptake over the BBB occurred in three different mouse models (E9: C57BL/6 mice, R3VQ: PS2APP mice, A2: rTg4510 mice). In addition, two of these VHHs (E9 and A2) were able to label intracellular antigens present in astrocytes (GFAP) and in neurons (p-Tau), respectively. These findings constitute strong evidence of transmigration of VHHs through the BBB and labeling of intracellular antigens, thus highlighting their potential for in vivo brain imaging.
- Monoclonal antibody production
- Nanobody production from llamas
- Rapid dipsticks test
- Cloning techniques
- Phage display
- Antibody characterization and conjugation
- In vivo assay
- Vandesquille M, Li T, Po C, Ganneau C , Lenormand P, Dudeffant C, Czech C, Grueninger F, Duyckaerts C, Delatour B, Dhenain M, Lafaye P, Bay S. Chemically-defined camelid antibody bioconjugate for the magnetic resonnace imaging of Alzheimer’s disease. MAbs, 2017, 9(6) : 1017-1028 doi: 10.1080/19420862.2017.1342914. PMID:28657418
- Magro L, Jacquelin B, Escadafal C, Garneret P, Kwasiborski A, Manuguerra J-C., Monti F, Sakunthabai A, Vanhomwegen J, Lafaye P, Tabeling P. Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics. Scientific Reports, 2017, 7: 1347 | DOI:10.1038/s41598-017-00758-9 PMID: 28465576
- Li T, Vandesquille M, Bay S, Dhenain M, Delatour B and Lafaye P. Selection of similar single domain antibodies from two immune VHH libraries obtained from two alpacas by using different selection methods. Immunology Letters, 2017, 188 : 89-95. doi: 10.1016/j.imlet.2017.07.001. PMID: 28690185
- Fédry J, Liu Y, Péhau-Arnaudet G, Pei J, Li W, Tortorici MA, Traincard F, Meola A, Bricogne G, Grishin NV, Snell WJ, Rey FA, Krey T. The ancient gamete fusogen HAP2 is a Eukaryotic class II fusion protein. Cell. 2017 Feb 23;168(5):904-915.e10. doi: 10.1016/j.cell.2017.01.024.
- Guillotte M, Nato F, Juillerat A, Hessel A, Marchand F, Lewit-Bentley A, Bentley GA, Vigan-Womas I, Mercereau-Puijalon O. Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin. Malar J. 2016 Jan 15;15(1):28.
- Boutet J, Blasco P, Guerreiro C, Thouron F, Dartevelle S, Nato F, Cañada FJ, Ardá A, Phalipon A, Jiménez-Barbero J, Mulard LA. Detailed Investigation of the Immunodominant Role of O-Antigen Stoichiometric O-Acetylation as Revealed by Chemical Synthesis, Immunochemistry, Solution Conformation and STD-NMR Spectroscopy for Shigella flexneri 3a. Chemistry. 2016 Jul 25;22(31):10892-911. doi: 10.1002/chem.201600567. Epub 2016 Jul 4.
- Li T, Vandesquille M, Koukouli F, Dudeffant C, Youssef I, Lenormand P, Ganneau C, Maskos U, Czech C, Grueninger F, Duyckaerts C, Dhenain M, Bay S, Delatour B, Lafaye P. Camelid single-domain antibodies: A versatile tool for in vivo imaging of extracellular and intracellular brain targets. Journal of Controlled Release 2016, 243 : 1-10 doi: 10.1016/j.jconrel.2016.09.019. PMID:27671875
- Agnememel A, Traincard F, Dartevelle S, Mulard L, Mahamane AE, Oukem-Boyer OO, Denizon M, Kacou-N Douba A, Dosso M, Gake B, Lombart JP, Taha MK. Development and evaluation of a dipstick diagnostic test for Neisseria meningitidis serogroup X. J Clin Microbiol., 2015 ; 53(2):449-54.
- Quaglio G, Goerens C, Putoto G, Rübig P, Lafaye P, Delaunois P, Zachariah R. : Ebola: lesson learnt and future challenges for Europe. The Lancet Infectious Diseases, 2015 ; pii: S1473- 3099(15)00361-8.
- Amyloid plaques detection by MRI with Gadolinium-VHH antibody conjugates. Vandesquille M., Li T.F., Koukouli F., Ganneau C., Maskos U., Czech C., Grueninger F., Duyckaerts C., Delatour B., Bay S., Lafaye P., Dhenain M. Neurodegenerative Diseases. 2015. 15(suppl 1): 896.
- Delmaghani S, Defourny J, Aghaie A, Beurg M, Dulon D, Thelen N, Perfettini I, Zelles T, Aller M, Meyer A, Emptoz A, Giraudet F, Leibovici M, Dartevelle S, Soubigou G, Thiry M, Vizi ES, Safieddine S, Hardelin JP, Avan P, Petit C. Hypervulnerability to Sound Exposure through Impaired Adaptive Proliferation of Peroxisomes. Cell. 2015 Nov 5;163(4):894-906.
- Delmaghani S, Defourny J, Aghaie A, Beurg M, Dulon D, Thelen N, Perfettini I, Zelles T, Aller M, Meyer A, Emptoz A, Giraudet F, Leibovici M, Dartevelle S, Soubigou G, Thiry M, Vizi ES, Safieddine S, Hardelin JP, Avan P, Petit C. Hypervulnerability to Sound Exposure through Impaired Adaptive Proliferation of Peroxisomes. Cell. 2015 Nov 5;163(4):894-906. doi: 10.1016/j.cell.2015.10.023.
- Gauthier C, Chassagne P, Theillet FX, Guerreiro C, Thouron F, Nato F, Delepierre M, Sansonetti PJ, Phalipon A, Mulard LA. Non-stoichiometric O-acetylation of Shigella flexneri 2a O-specific polysaccharide: synthesis and antigenicity. Org Biomol Chem., 2014 ; 12(24) : 4218-32.
- Collard JM, Wang X, Mahamane AE, Idi I, Issaka B, Ousseni M, Mayer LW, Nato F, Moulia-Pelat JP. A five-year field assessment of rapid diagnostic tests for meningococcal meningitis in Niger by using the combination of conventional and real-time PCR assays as a gold standard.Trans R Soc Trop Med Hyg., 2014 ; 108(1):6-12
- Goarant C, Bourhy P, D'Ortenzio E, Dartevelle S, Mauron C, Soupé-Gilbert ME, Bruyère-Ostells L, Gourinat AC, Picardeau M, Nato F, Chanteau S. Sensitivity and Specificity of a New Vertical Flow Rapid Diagnostic Test for the Serodiagnosis of Human Leptospirosis. PLoS Negl Trop Dis., 2013 ; 7(6):e2289.
- Zarantonelli ML, Skoczynska A, Antignac A, El Ghachi M, Deghmane AE, Szatanik M, Mulet C, Werts C, Peduto L, d'Andon MF, Thouron F, Nato F, Lebourhis L, Philpott DJ, Girardin SE, Vives FL, Sansonetti P, Eberl G, Pedron T, Taha MK, Boneca IG. Penicillin resistance compromises Nod1-dependent proinflammatory activity and virulence fitness of Neisseria meningitidis. Cell Host Microbe, 2013 ; 13 : 735-45.
- Terrade A, Collard JM, Nato F, Taha MK. Laboratory evaluation of a rapid diagnostic test for Neisseria meningitidis serogroup A. Trans R Soc Trop Med Hyg., 2013 ; 107 : 460-1.
- Tarr A W, Lafaye P, Meredith L, Damier-Piolle L, Urbanowicz R A, Meola A, Jestin J-L , Brown R, McKeating J A, Rey F A, Ball J-K , Krey T. A recombinant nanobody blocks hepatitis C virus entry and cell-to-cell transmission. Hepatology, 2013; 58(3): 932-939
- Duran C., Nato F., Dartevelle S., Phuong L.N.T., Taneja N., Ungeheuer M-N., Soza G., Anderson L., Benadof D., Zamorano A., Diep T.T., Nguten T.Q., Nguyen V.H., Ottone C., Bégaud E., Pahil S., Prado V., Sansonetti P., Germani Y. Rapid Diagnosis of diarrhea caused by Shigella sonnei using Dipsticks; comparison of rectal swabs, direct stool and stool culture. PLOS One, 2013; 8(11): e80267.
- PCT EP 2014/074066. High-affinity monoclonal anti-Strep-Tag antibody C23.21. Girard-blanc C., Krey T., Vasiliauskaite I., Nato F., Lafaye P., Dartevelle S., Rey F.
- EP 12187318.6 - 04/10/2012. New neutralizing antibodies directed against Hepatitis C virus. Krey T., Tarr A., Lafaye P., Ball J., Jestin, J-L., Rey F. EP12/187318.6 - 17/12/2012
- EP14306389.9 - PCT/EP2015/070669. Polypeptide-base carrier for the delivery of molecules across blood-brain barrier. Prehaud C, Lafaye P, Afonso P, Ceccaldi PE, Lafon M
- EP 14306388- PCT/EP2015/070669. Nanobodies suitable for neuron regeneration therapy. Prehaud C, Lafaye P, Lafon M
- PCT/FR2012/052771 – 3/11/2012. Use of anti-FcγRI and/or anti-FcγRIIA antibodies for treating arthritis ;, inflammation, thrombocytopenia and allergic shock. Jonsson F., Mancardi D., Lafaye P., Bruhns P.,
- EP 13306553.2 - 13/11/2013. Camelid single-domain antibody directed against amyloid beta and methods for producing conjugates thereof. Lafaye P., Bay S., Czech C., Delatour B., Dhenain M., Duyckaerts C., Grueninger F., Li T., Vandesquille M.
- EP 13306554.0 - 13/11/2013. Oligopeptides and methods for producing conjugates thereof. Lafaye P., Bay S., Czech C., Delatour B., Dhenain M., Duyckaerts C., Grueninger F., Li T., Vandesquille M.
- DI 2014-01 - EP 14152928.9 - 28/01/2014. Camelid single-domain antibodies directed against phosphorylated Tau proteins and methods for producing conjugates thereof. Lafaye P., Bay S., Czech C., Delatour B., Dhenain M., Duyckaerts C., Grueninger F., Li T., Vandesquille M.
- CroatiaStipan JonjicUniversity of Rijeka
- DenmarkJakub Z. KaczmarekSanovo Biotech A/S
- FranceAndré PèlegrinInstitut de Recherche en Cancérologie de Montpellier, IRCM
- FranceArmand BensussanHôpital Saint Louis
- FrancePierre LafayeInstitut Pasteur
- GermanyFriedrich Koch-NolteUniversity Medical Center Hamburg-Eppendorf
- HungaryJohanna SymmonsEnglish & Scientific Consulting Kft. (SCICONS)
- HungaryPeter BaloghClinical Center, University of Pecs
- ItalyFabio MalavasiUniversity of Torino Medical School
- RussiaAlexander FilatovInstitute of Immunology
- RussiaAlexander TaraninInstitute of Molecular and Cellular Biology
- SloveniaVladka Čurin ŠerbecBlood Trasfusion Centre of Slovenia
- SpainGiovanna RoncadorCentro Nacional de Investigaciones Oncológicas
- SpainJosé I. Rodríguez BarbosaEdificio Nuevos Institutos de Investigación
- SpainLeonor KremerCentro Nacional de Biotecnología/CSIC
- SpainNúria PascualInstitute for Advanced Chemistry of Catalonia (IQAC-CSIC)
- SpainPablo EngelFaculty of Medicine and Medical Sciences, University of Barcelona
- United KingdomUniversity of OxfordLRF Haemato-oncology Group
- United KingdomVertebrate Antibodies Limited (VAL)
- United KingdomKing's College LondonSt. John's Institute of Dermatology