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Núria Pascual

Name of the laboratory

Cabs, CSIC, CIBER-BBN, Nan Biosis
Custom Antibody Service (CAbS)
Institute for Advanced Chemistry of Catalonia (IQAC-CSIC)
IQAC-CSIC, CIBER-BBN
Jordi Girona, 18-26, 08034-Barcelona, Spain
+34 93 400 61 00 Ext.: 5116

Members of the laboratory

Scientific Coordinator
Dr. Núria Pascual nuria.pascual@cid.csic.es
Scientific Director
Prof. M.-Pilar Marco pilar.marco@cid.csic.es
Technicians
Idoia Camí
Andrea Bastias
Lidia Hinojosa

Laboratory activity

The Custom Antibody Service (CAbS) is a joint facility established under the umbrella of the Institute for Advanced Chemistry of Catalonia of the Spanish Council for Scientific Research (IQAC-CSIC) and the Networking Research Center for Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). CAbS aim is to provide services and scientific advice, in relation to the production and use of specific antibodies and biomolecular probes to public and private research centers and companies. Antibodies for proteins, peptides, small organic molecules or other antigens can be produced using standard or customized protocols according to each customer's needs.

CAbS is managed by the scientific team of the Nb4D group (Nb4D), recognized for their expertise in hapten synthesis, antibody production and immunochemical method development. Nb4D provides all the necessary advice and support to each project.

CAbS forms part of the NANBIOSIS (infrastructure of production and characterization of nanomaterials, biomaterials and biomedical systems). NANBIOSIS is one of the 29 ICTS (Singular Scientific Technological Infrastructures) that have been recently recognized by the Spanish Ministry for Economy and Competitivity.

Research activities

The CAbS facility aim is to offer a high quality service for customized monoclonal and polyclonal antibody production against many types of antigens including proteins, peptides or small organic molecules. Moreover, CAbS can offer additional services related to the preparation of immunoreagents and development of immunochemical methods and protocols.

CAbS has extensive experience and successful records of developing antibodies against small molecule antigens for research, diagnostics, environmental monitoring, food testing and drug abuse testing. Cabs has the expertise (10 patents and some of them are licensed to companies) and infrastructure to the hapten design and synthesis, to provide the small molecule antigens with an optimized immunization protocol and screening procedures. We are able to modulate the immunoassay features by appropriate immunizing and competitor hapten design.

Techniques available

  • Hapten design and synthesis: Chemical synthesis, purification and characterization of hapten.
  • Preparation of bioconjugates: labelled antibodies, haptenized proteins and enzymes, biotynilated and fluorescent probes, gold nanoparticle conjugates, etc
  • Polyclonal Antibody Production (goat, rabbit, rat)
  • Monoclonal Antibody Development (mouse, rat)
  • Hybridoma cell culture antibody production
  • Antibody purification
  • Preparation of antibody fragments.
  • Development of immunochemical methods: ELISA, immunoaffinity columns, etc.

Publications (2021-present)

  • V. Garzón, D.G.-Pinacho, J.-P. Salvador, M.-P. Marco, R.-H. Bustos. Advanced Biosensing Strategies for Last-Line Antibiotics vancomycin, colistin, daptomycin and meropenem: Comparative Analysis of Electrochemical and Optical Detection Methods. Antibiotics, 2026. DOI: xxxx
  • F. Gkartziou, V. Petegnief, M. Kannavou, C. Flavián-Lázaro, N. Pascual, L. Márquez, M.-P. Marco, A. M. Planas, S. G. Antimisiaris. Development of Immunoliposomes with E-Selectin affinity to target endothelial inflammation: Direct comparison of antibody types. J. Liposome Res., 2026. DOI: xxxx
  • B. Rodríguez, T. Posvai, L. Vilaplana, M.-P. Marco. Anti-pyocyanin Antibody Exhibits Cytotoxicity Protective Effects on Macrophages: A Promising Innovative Therapeutic Approach for Pseudomonas aeruginosa Infections. ACS Pharmacol. Transl. Sci., 8(12), 4268-4283, 2025. DOI: 10.1021/acsptsci.5c00187
  • M. Gutiérrez-Capitán, E. Balada, A. Aviñó, L. Vilaplana, R. Galve, A. Lacoma, A. Baldi, A. Alcamí, V. Noe, C. Ciudad, R. Eritja, M.-P. Marco, C. Fernández-Sánchez. Unravelling the Amplification-Free Quantitative Detection of Viral RNA in Nasopharyngeal Swab Samples Using a Compact Electrochemical Rapid Test Device. Anal. Chem., 97(22), 11863-11873, 2025. DOI: 10.1021/acs.analchem.5c01605
  • J. Raya, E. Montagut, M.-P. Marco. Analysing the integrated quorum sensing (Iqs) system and its potential role in Pseudomonas aeruginosa pathogenesis. Front. cell. infect. microbiol., 15:1575421, 2025. DOI: 10.3389/fcimb.2025.1575421
  • A. Narváez, J. Jiménez, M. Rodríguez-Núñez, M. Torre, E. Carro, M.-P. Marco, E. Domínguez. A Fast Immunosensor Based on Biohybrid Self-Assembled Nanostructures for the Detection of KYNA as a Cerebrospinal Fluid Biomarker for Alzehimer's Disease. ACS Meas. Sci. Au., 5(2), 242-249, 2025. DOI: 10.1021/acsmeasuresciau.4c00102
  • J. Guercetti, M. Alorda, L. Sappia, R. Galve, M. Duran-Corbera, D. Pulido, G. Berardi, M. Royo, A. Lacoma, J. Muñoz, E. Padilla, S. Castañeda, E. Sendra, J.-P. Horcajada, A. Gutiérrez-Gálvez, S. Marco, J.-P. Salvador, M.-P. Marco. Immuno-uSARS2 chip: a peptide-based microarray to assess COVID-19 prognosis based on immunological fingerprints. ACS Pharmacol. Transl. Sci., 8(3), 871-884, 2025. DOI: 10.1021/acsptsci.4c00727
  • N. Roson-Calero, M. A. Gomis Font, A. Ruiz-Soriano, X. Just-Baringo, M. E. Pachón-Ibáñez, J.-P. Salvador, M.-P. Marco, E. Giralt, A. Oliver, C. Ballesté-Delpierre, J. Vila. In vitro potentiation of tetracyclines in Pseudomonas aeruginosa by RW01, a new cyclic peptide. Antimicrob Agents Chemother, 69:e01459-24, 2024. DOI: 10.1128/aac.01459-24
  • B. Rodríguez-Urretavizcaya, L. Vilaplana, M.-P. Marco. Strategies for Quorum Sensing inhibition as a tool for controlling Pseudomonas aeruginosa infections. Int. J. Antimicrob. Agents, 64(5), 107323, 2024. DOI: 10.1016/j.ijantimicag.2024.107323
  • J. Guercetti, N. Pascual, A. Aviñó, R. Eritja, J.-P. Salvador, M.-P. Marco. DNA-directed immobilization fluorescent immunoarray for multiplexed antibiotic residue determination in milk. Anal. Bioanal. Chem., 416, 7325-7336, 2024. DOI: 10.1007/s00216-024-05481-9
  • V. Garzon, J.-P. Salvador, M.-P. Marco, D. G.-Pinacho, R.-H. Bustos. Production of antibodies against the colistin, vancomycin, daptomycin and meropenem antibiotics and characterization using the ELISA immunoassay: An approach for its use in Therapeutic Drug Monitoring. Antibiotics, 13(7), 600, 2024. DOI: 10.3390/antibiotics13070600
  • G. Colom, A. Hernández-Albors, J. Barallat, A. Galan, A. Bayes-Genis, J.-P. Salvador, M.-P. Marco. A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers. Microchim Acta, 191, 54, 2024. DOI: 10.1007/s00604-023-06119-w
  • M. Gutiérrez-Capitán, A. Sanchís, E. Carvalho, A. Baldi, L. Vilaplana, V. Cardoso, A. Calleja, M. Wei, R. de la Rica, J. Hoyo, A. Bassegoda, T. Tzanov, M.-P. Marco, S. Lanceros-Méndez, C. Fernández-Sánchez. Engineering a Point-of-Care Paper-Microfluidic Electrochemical Device applied to the Multiplexed Quantitative Detection of Biomarkers in Sputum. ACS Sensors, 8(8), 3032-3042, 2023. DOI: 10.1021/acssensors.3c00523
  • C. Adrover-Jaume, A. Clemente, B. Rodríguez-Urretavizcaya, L. Vilaplana, M.-Pilar Marco, E. Rojo-Molinero, A. Oliver, R. de la Rica. A paper biosensor for overcoming matrix effects interfering with the detection of sputum pyocyanin with competitive immunoassays. Microchim Acta, 190, 441, 2023. DOI: 10.1007/s00604-023-06017-1
  • J. Guercetti, J.-P. Salvador, M.-P. Marco. Bioreceptors for smartphone-based food contaminants detection. (BOOK CHAPTER) DOI: 10.1016/bs.coac.2022.11.001
  • E. Montagut, J. Raya, M.-T. Martín Gómez, L. Vilaplana, B. Rodríguez-Urretavizcaya, M.-P. Marco. An Immunochemical Approach to detect the Quorum Sensing-Regulated Virulence Factor 2-Heptyl-4-Quinoline N-Oxide (HQNO) produced by Pseudomonas aeruginosa Clinical Isolates. Microbiol. Spect., 10(4), 1-12, 2022. DOI: 10.1128/spectrum.01073-21
  • S. Jafari, L. Burr, D. Migliorelli, R. Galve, M.-P. Marco, K. Campbell, C. Elliott, M. Suman, S. J. Sturla, S. Generelli. Smartphone-based magneto-immunosensor on carbon black modified screen-printed electrodes for point-of-need detection of aflatoxin B1 in cereals. Anal. Chim. Acta, 1221, 340118, 2022. DOI: 10.1016/j.aca.2022.340118
  • A. Aviñó, C. Cuestas-Ayllón, M. Gutiérrez-Capitán, L. Vilaplana, V. Grazu, V. Noe, E. Balada, A. Baldi, A. J. Félix, E. Aubets, S. Valiuska, A. Domínguez, R. Gargallo, R. Eritja, M.-P. Marco, C. Fernández-Sánchez, J. Martínez de la Fuente, C. J. Ciudad. Detection of SARS-CoV-2 virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA). Int. J. Mol. Sci., 23(23), 15258, 2022. DOI: 10.3390/ijms232315258
  • L. Xu, X. Zhang, A.M.A. El-Aty, Y. Wang, Z. Cao, H. Jia, J.-P. Salvador, A. Hacimuftuoglu, X. Cui, Y. Zhang, K. Wang, Y. She, F. Jin, L. Zheng, B. Pujia, J. Wang, M. Jin, B.D. Hammock. A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching. J. Pharm. Anal., 12(4), 627, 2022. DOI: 10.1016/j.jpha.2022.05.004
  • J. Dietvorst, A. Ferrer-Vilanova, S. Narayana Iyengar, A. Russom, N. Vigués, J. Mas, Ll. Vilaplana, M.-P. Marco, G. Guirado, X. Muñoz-Berbel. Bacteria detection at single-cell level through a cyanotype-based photochemical reaction. Anal. Chem., 94(2), 787-792, 2022. DOI: 10.1021/acs.analchem.1c03326
  • J.-P. Salvador, T. Brettschneider, C. Dorrer, M.-P. Marco. Development of a fluorescent microfluidic device based on antibody microarray read-out for therapeutic drug monitoring of acenocoumarol. Front. Bioeng. Biotechnol., 10, 848501, 2022. DOI: 10.3389/fbioe.2022.848501
  • M. Burdó-Masferrer, M. Díaz-González, A. Sanchis, A. Calleja, M.-Pilar Marco, C. Fernández-Sánchez, A. Baldi. Compact microfluidic platform with LED light actuated valves for enzyme-linked immunosorbent assay automation. Biosensors, 12(5), 280, 2022. DOI: 10.3390/bios12050280
  • E. Montagut, G. Acosta, F. Albericio, M. Royo, G. Godoy-Tena, A. Lacoma, C. Prat, J.-P. Salvador, M.-P. Marco. Direct Quantitative Immunochemical Analysis of the Autoinducer Peptide IV (AIP-IV) for Diagnosing and Stratifying Staphylococcus aureus infections. ACS Infect. Dis., 8(3), 645-656, 2022. DOI: 10.1021/acsinfecdis.1c00670
  • J.-P. Salvador, M.-P. Marco, G. Saviozzi, C. Laschi, F. Arreza, F. Palacio, M. Lopez. Portable flow multiplexing device for continuous, in situ biodetection of environmental contaminants. Sens. Bio-Sens. Res., 37, 100505, 2022. DOI: 10.1016/j.sbsr.2022.100505
  • E. J. Montagut, M.T. Martin-Gómez, M.-P. Marco. An Immunochemical Approach to Quantify and Assess the Potential Value of Pseudomonas Quinolone Signal (PQS) as a Biomarker of Infection. Anal. Chem., 93(11), 4859-4866, 2021. DOI: 10.1021/acs.analchem.0c04731
  • J. Marrugo-Ramírez, M. Rodríguez-Núñez, M.-P. Marco, M. Mir, J. Samitier. Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer's Disease. Biosensors, 11(1), 20, 2021. DOI: 10.3390/bios11010020
  • B. Rodriguez-Urretavizcaya, N. Pascual, C. Pastells, M. T. Martin-Gomez, Ll. Vilaplana, M.-P. Marco. Diagnostic and Stratification of Pseudomonas aeruginosa Infected Patients by Immunochemical Quantitative Determination of Pyocyanin from Clinical Bacterial Isolates. Frontiers in Cell. Infect. Microbiol., 11, 786929, 2021. DOI: 10.3389/fcimb.2021.786929
  • X. Cui, A. M. A. El-Aty, C. Zhang, L. Xu, H. Liu, H. Jia, Y. Wang, Z. Cao, J.-P. Salvador, Y. She, F. Jin, J. Wang, M. Jin, B. D. Hammock. Enhanced Bio-Barcode Immunoassay Using Droplet Digital PCR for Multiplex Detection of Organophosphate Pesticides. J. Agr. Food Chem., 69(37), 11131-11141, 2021. DOI: 10.1021/acs.jafc.1c03216
  • S. Jafari, J. Guercetti, A. Geballa-Koukoula, A.S. Tsagkaris, J.L.D. Nelis, M.-P. Marco, J.-P. Salvador, A. Gerssen, J. Hajslova, C. Elliott, K. Campbell, D. Migliorelli, L. Burr, S. Generelli, M.W.F. Nielen, S.J. Sturla. ASSURED Point-of-Need food safety screening: a critical assessment of portable food analyzers. Foods, 10(6), 1399, 2021. DOI: 10.3390/foods10061399
  • E. J. Montagut, M.-P. Marco. Biological and Clinical Significance of Quorum Sensing Alkylquinolones: Current Analytical and Bioanalytical Methods for their Quantification. Anal. Bioanal. Chem., 413(18), 4599-4618, 2021. DOI: 10.1007/s00216-021-03356-x
  • J. L. D. Nelis, J.-P. Salvador, M.-P. Marco, C. T. Elliott, K. Campbell. A plasmonic biosensor array exploiting plasmon coupling between gold nanorods and spheres for domoic acid detection via two methods. Spectrochim. Acta A, 252, 119473, 2021. DOI: 10.1016/j.saa.2021.119473