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Núria Pascual

Name of the laboratory

Cabs, CSIC, CIBER-BBN, Nan Biosis
Custom Antibody Service (CAbS)
Institute for Advanced Chemistry of Catalonia (IQAC-CSIC)
IQAC-CSIC, CIBER-BBN
Jordi Girona, 18-26, 08034-Barcelona, Spain
+34 93 400 61 00 Ext.: 5116

Members of the laboratory

Scientific Coordinator
Dr. Núria Pascual nuria.pascual@cid.csic.es
Scientific Director
Prof. M.-Pilar Marco pilar.marco@cid.csic.es
Technicians
Melek Denizli
Camilo Perales
Astrid Garcia

Laboratory activity

The Custom Antibody Service (CAbS) is a joint facility established under the umbrella of the Institute for Advanced Chemistry of Catalonia of the Spanish Council for Scientific Research (IQAC-CSIC) and the Networking Research Center for Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). CAbS aim is to provide services and scientific advice, in relation to the production and use of specific antibodies and biomolecular probes to public and private research centers and companies. Antibodies for proteins, peptides, small organic molecules or other antigens can be produced using standard or customized protocols according to each customer's needs.

CAbS is managed by the scientific team of the Nb4D group (Nb4D), recognized for their expertise in hapten synthesis, antibody production and immunochemical method development. Nb4D provides all the necessary advice and support to each project.

CAbS forms part of the NANBIOSIS (infrastructure of production and characterization of nanomaterials, biomaterials and biomedical systems). NANBIOSIS is one of the 29 ICTS (Singular Scientific Technological Infrastructures) that have been recently recognized by the Spanish Ministry for Economy and Competitivity.

Research activities

The CAbS facility aim is to offer a high quality service for customized monoclonal and polyclonal antibody production against many types of antigens including proteins, peptides or small organic molecules. Moreover, CAbS can offer additional services related to the preparation of immunoreagents and development of immunochemical methods and protocols.

CAbS has extensive experience and successful records of developing antibodies against small molecule antigens for research, diagnostics, environmental monitoring, food testing and drug abuse testing. Cabs has the expertise (10 patents and some of them are licensed to companies) and infrastructure to the hapten design and synthesis, to provide the small molecule antigens with an optimized immunization protocol and screening procedures. We are able to modulate the immunoassay features by appropriate immunizing and competitor hapten design.

Techniques available

  • Hapten design and synthesis: Chemical synthesis, purification and characterization of hapten.
  • Preparation of bioconjugates: labelled antibodies, haptenized proteins and enzymes, biotynilated and fluorescent probes, gold nanoparticle conjugates, etc
  • Polyclonal Antibody Production (goat, rabbit, rat)
  • Monoclonal Antibody Development (mouse, rat)
  • Hybridoma cell culture antibody production
  • Antibody purification
  • Preparation of antibody fragments.
  • Antibody characterization (Isotyping)
  • Development of immunochemical methods: ELISA, immunoaffinity columns, etc.
  • Mycoplasma testing

Publications (2016-present)

  • Sonia Herranz, Marzia Marciello, María -P. Marco, José L. Garcia-Fierro, José M. Guisan and María C. Moreno-Bondi Sensors and Actuators B: Chemical 257, 256-262, 2018.
  • Sonia Matas, Marta Broto, Mercè Corominas, Ramon Lleonart, Ruth Babington, M-Pilar Marco, Roger Galve. Immediate hypersensitivity to penicillins. Identification of a new antigenic determinant. Journal of pharmaceutical and biomedical analysis 148, 17-23. 2018
  • M Broto, JP Salvador, R Galve, MP Marco. Biobarcode assay for the oral anticoagulant acenocoumarol. Talanta 178, 308-314, 2018.
  • Broto, M; Mc Cabe, Rita, Galve, Roger, Marco, M.P. A high throughput immunoassay for the therapeutic drug monitoring of tegafur. Analyst2017, 142, 2404-2410. URL
  • Broto, M; Galve, R; Marco, M.P. Bioanalytical methods for cytostatic therapeutic drug monitoring and occupational exposure assessment. Trends in Analytical Chemistry 2017, 93, 152-170.
  • Castellarnau, M.; Ramon-Azcon, J.; Gonzalez-Quinteiro, Y.; Lopez, J. F.; Grimalt, J. O.; Marco, M. P.; Nieuwenhuijsen, M.; Picado, A., Assessment of analytical methods to determine pyrethroids content of bednets. Tropical Medicine & International Health : TM & IH 2017, 22 (1), 41-51.
  • Hassani, N. E. A. E.; Baraket, A.; Neto, E. T. T.; Lee, M.; Salvador, J. P.; Marco, M. P.; Bausells, J.; Bari, N. E.; Bouchikhi, B.; Elaissari, A.; Errachid, A.; Zine, N., Novel strategy for sulfapyridine detection using a fully integrated electrochemical Bio-MEMS: Application to honey analysis. Biosensors and Bioelectronics 2017, 93, 282-288
  • Tort, N.; Salvador, J. P.; Marco, M. P., Multimodal plasmonic biosensing nanostructures prepared by DNA-directed immobilization of multifunctional DNA-gold nanoparticles. Biosensors and Bioelectronics 2017, 90, 13-22.
  • Pastells, C.; Marco, M. P.; Merino, D.; Loza-Alvarez, P.; Pasquardini, L.; Lunelli, L.; Pederzolli, C.; Daldosso, N.; Farnesi, D.; Berneschi, S.; Righini, G. C.; Quercioli, F.; Nunzi Conti, G.; Soria, S., Two photon versus one photon fluorescence excitation in whispering gallery mode microresonators. Journal of Luminescence 2016, 170, Part 3, 860-865.
  • Pastells, C.; Pascual, N.; Sánchez-Baeza, F.; Marco, M.-P., Immunochemical Determination of Pyocyanin and 1-Hydroxyphenazine as Potential Biomarkers of Pseudomonas aeruginosa Infections. Analytical Chemistry 2016, in press.
  • Roncador, G.; Engel, P.; Maestre, L.; Anderson, A. P.; Cordell, J. L.; Cragg, M. S.; Šerbec, V. Č.; Jones, M.; Lisnic, V. J.; Kremer, L.; Li, D.; Koch-Nolte, F.; Pascual, N.; Rodríguez-Barbosa, J.-I.; Torensma, R.; Turley, H.; Pulford, K.; Banham, A. H., The European antibody network's practical guide to finding and validating suitable antibodies for research. mAbs 2016, 8 (1), 27-36.
  • Sanmartí-Espinal, M.; Galve, R.; Iavicoli, P.; Persuy, M.-A.; Pajot-Augy, E.; Marco, M. P.; Samitier, J., Immunochemical strategy for quantification of G-coupled olfactory receptor proteins on natural nanovesicles. Colloids and Surfaces B: Biointerfaces 2016, 139, 269-276.
  • Valera, E.; Hernández-Albors, A.; Marco, M. P., Electrochemical coding strategies using metallic nanoprobes for biosensing applications. TrAC Trends in Analytical Chemistry 2016, in press